Zeiss Celldiscoverer 7 fully automated widefield and LSM confocal microscope
- Contrast techniques brightfield, oblique, adaptive phase contrast
- WF LEDs: 385, 420, 470, 520, 567, 590, 625
- WF emission filters: QBP 425 / 30+514 / 30+592 / 25+709 / 100; TBP 467 / 24+555 / 25+687 / 145
- LSM lasers: 405, 488, 561, 640
- LSM detectors: 2x GaAsp-PMT; 1x Airyscan detector for higher sensitivity or multiplex detection
- Objective Plan-Apochromat 5x/0.35 WD 5.10 Dry; Magnification options (with tubelens): 2.5×/0.12, 5x/0.25, 10x/0.35
- Objective Plan-Apochromat 20x/0.7 WD 2.20 Dry autocorrection; Magnification options (with tubelens): 10×/0.35, 20x/0.7, 40x/0.7
- Objective Plan-Apochromat 50x/1.2 WD 0.84 Water autoimmersion autocorrection; Magnification options (with tubelens): 25×/1.2, 50x/1.2, 100x/1.2
- Temperature and CO2 control
Vala Sciences Kinetic Image Cytometer (in collaboration with Circulatory Health Research Centre)
Liquid handling
- Opentrons liquid handling workstation with 20ul and 300ul multichannel (8x) pipettes; heating cooling devices (2x); clean air downflow (HEPA filter)
- Dispendix I.DOT non-contact dispenser for printing compounds etc.
- BioTek MultifloFX liquid dispenser
- Blue Cat Bio Centrifugal BlueWasher for plate washing
Storage hotels
- LiCONic automated CO2 incubator (37° Celcius) with 66 slots
- Plate hotel (room temp) with 12 slots
Miscellaneous
- Delidder station (to automatically remove lids from assay plates)
- Barcode reader
Rosie is located at Hubrecht E.4.38, Uppsalalaan 8, 3584 CT, Utrecht.
Direct booking is not available, access to the equipment is offered as part of a screening and automation service.
