Library Preperation

Convert your sample into sequence libraries.

We facilitate the following library preparation methods using standard Illumina reagents. For turn around times, see our FAQ on the USEQ website.

Available kits:

• TruSeq DNA Nano LT
• TruSeq Stranded total RNA, ribo zero for human/mouse/rat
• TruSeq Stranded mRNA polyA

A maximum of 24 (single index) or 96 (dual index) TruSeq indexes is available. Please contact us if you would like to use custom indexes. Bioinformatics demultiplexing service is supported for a restricted set of indexes.

Sample requirements

All concentrations should be measured with a fluorometric quantitation analysis (e.g. Qubit).

For DNA library preparation:

• a minimum of 50 μl at a minimal concentration of 4 ng/μl.

For RNA library preparation:

• RiboZero depletion: a minimum of 12 μl at a minimal concentration of 17 ng/μl.
• PolyA enrichment: a minimum of 50 μl at a minimal concentration of 4 ng/μl.
• RNA quality should be at least RIN 8 (RNA Integrity Number)

We strongly advise to include DNase treatment during the RNA isolation process.

Requester is responsible for collection of primary sample. Note that samples will be stored for a maximum of 2 months and subsequently discarded without further notice.