Label-free MALDI mass spectrometry imaging
With timsTOF, Bruker introduces the next generation of ion mobility mass spectrometry. Trapped ion mobility spectrometry (TIMS) unlocks an additional dimension of separation and delivers revolutionary improvements in enhanced specificity and high sensitivity.
The timsTOF fleX brings Bruker’s powerful MALDI technology to the portfolio, adding a high-resolution spatial dimension and enabling the field of spatial omics on one platform for the first time. The timsTOF fleX with PASEF technology enables fast scanning speeds.
The newly developed smartbeam 3D laser fires at a repetition rate of up to 10 kHz and features a laser diameter of 5 µm. The 10 kHz smartbeam 3D technology allows for up to 20 times faster MALDI tissue imaging in MS mode. The laser can move independently from the (continuously moving) sample stage to scan the full area of each pixel and achieve high pixel rates. This produces truly square pixels, utilizing all of the available sample area for maximum sensitivity and pixel-to-pixel reproducibility for routine imaging at 20 µm spatial resolution.
Facilities where you can find this equipment:
| Description | Specification | Description | Specification |
|---|---|---|---|
| resolution | 60,000 | source | ESI and MALDI |
| scan speed | 1 -> 100 Hz (MS & PASEF) | acquisition | PASEF |
| lateral spatial resolution | 20 µm | MALDI mode (rel. intensity) | 1 (Cholesterol) 1 (Vitamin D) |
Bruker timsTOF fleX
