Utrecht Sequencing Facility (USEQ)

Equipment & Application

USEQ operates a broad range of sequencing platforms, each with unique characteristics. Technical and cost characteristics determine the best fit for your experiment. Service fees include costs for chemicals, operator time, quality control, maintenance, lab information management system (LIMS) and troubleshooting.

Platform & Configurations

Currently, we operate the following Illumina platforms and configurations:

  • 1x NextSeq500
  • 2x NextSeq2000
  • 1x iSeq100
  • 2x NovaSeq 6000 (external)
  • Nanopore MinIon
  • Nanopore GridIon X5
  • Nanopore ProMethion
  • Nanopore PromethIon (1 machines)

On this page you can find an overview per platform of the most widely used run modi, configurations and expected output*. Other run modi and custom recipes are possible as well, upon consultation with USEQ.

* Please note that high quality of sequence libraries is crucial in order to obtain maximum output. For samples submitted as ready to sequence libraries, USEQ cannot take any responsibility for data quality and quantity.

DNA / RNA isolation

From cells, blood, tissue etc

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illumina iSeq 100

Applications: small scale amplicon sequencing,Novaseq pooling and rebalancing.

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Illumina NextSeq2000

Applications: genome, exome, transcriptome sequencing.

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Illumina NextSeq500

Applications: genome, exome, transcriptome sequencing.

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illumina Novaseq 6000

Applications: large scale genome, exome, transcriptome sequencing.

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illumina NovaSeq X

Applications: large scale genome, exome, transcriptome sequencing.

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Library Preperation

Convert your sample into sequence libraries

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Nanostring CosMx Spatial Molecular Imager

Targeted spatial transcriptomics and proteomics

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Nanostring GeoMx Digital Spatial Profiler

Untargeted spatial transcriptomics and proteomics

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Next Generation Sequencing

NGS platforms and additional services

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Oxford Nanopore Sequencing

Using the MinION platform

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Whole Genome Sequencing

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