The Utrecht Sequencing Facility provides support for Next Generation Sequencing (NGS) based research
We provide support for a broad range of services, including DNA/RNA isolation, sample preparation, Next Generation Sequencing, SNP fingerprinting, Spatial Omics and basic data analysis.
A comprehensive state-of-the-art infrastructure is available, including NGS sequencers, liquid handling robotics, bioinformatics tools and ICT.
You are welcome to take a look at our standard services or contact us for any customizations.
The Utrecht Sequencing Facility is sponsored by the University Medical Center Utrecht and the NWO infrastructure grant X-omics.
Please contact us to discuss the possibilities for your research.
Obtaining high quality sequencing data starts with high quality DNA and RNA. We use the Qiagen QiaSymphony SP for its proven robust high quality automated isolation of whole tissue, cells, organoids, blood. Standard services include human and mouse samples. Please contact us to discuss possibilities for samples of other species. For turn around times, see our FAQ on the USEQ website.
We request samples to be submitted as either snapfrozen tissue, dry cell / organoid pellet, or dissolved in 200 ul ATL buffer (for DNA), dissolved in 400 ul RLT+ buffer (for RNA, stored at -80C).
Convert your sample into sequence libraries
We facilitate the following library preparation methods using standard Illumina reagents. For turn around times, see our FAQ on the USEQ website.
Available kits:
A maximum of 24 (single index) or 96 (dual index) TruSeq indexes is available. Please contact us if you would like to use custom indexes. Bioinformatics demultiplexing service is supported for a restricted set of indexes.
All concentrations should be measured with a fluorometric quantitation analysis (e.g. Qubit).
For DNA library preparation:
For RNA library preparation:
Requester is responsible for collection of primary sample. Note that samples will be stored for a maximum of 2 months and subsequently discarded without further notice.
USEQ operates a broad range of sequencing platforms, each with unique characteristics. Technical and cost characteristics determine the best fit for your experiment. Service fees include costs for chemicals, operator time, quality control, maintenance, lab information management system (LIMS) and troubleshooting.
Currently, we operate the following Illumina platforms and configurations:
Determine RNA or protein profiles based on morphology from small cell populations to within single cells
Current techniques offer a trade-off between spatial data and in-depth expression data. Bulk sequencing, for instance, generates in-depth data, but often it tells us little about where these cells were situated in the original tissue. Techniques like single molecule FISH and immunohistochemistry offer us spatial data, but only have a limited number of targets. USEQ offers a new technique called Nanostring GeoMX (Cell population based) & Nanostring CosMX (single cell or subcellular), which combines the spatial data with RNA or protein expression profiles. You provide the tissue of your choice and select regions that interest you. This can be done with Fresh Frozen or FFPE standard microscopy slides. Please call us for a detailed consult.