Proteomics

Cost breakdown

We are a non-commercial facility for the greater Oncode/life science community.

Costs

To cover depreciation of the instrumentation, maintenance service contracts and local technical support, the facility charges a non-commercial price of €50 per hour of measurement time, plus half an hour of loading time per sample. When a certain experiment necessitates the use of expensive reagents, (such as TMT labels or UPS2 spike-in for absolute protein quantification) these will also be charged.

Joint publications

Furthermore, since experiments are done in collaboration with Proteomics4Oncode, these will result in joint publications between the facility and the ‘customer’.

Each experiment is unique. But, to give you a sense of cost, we’ve supplied a few examples:

Example #1: GFP affinity enrichment of protein X using label-free quantification — €800,-

4 controls and 4 specific purifications
- 8 samples total
1 hour run hour loading per sample
- 8 [samples] x 1 = 8 hours of running time
8 hour running time x € 100 hourly rate
- Totals € 800,- running cost

Example #2: Whole proteome comparison between 2 conditions using label-free quantification — Deep Proteome — €1800,-

3 x condition A and 3 x condition B
- 6 samples total
Per sample: fractionation into 3 fractions for deeper proteome
- 6 [samples] x 3 runs = 18 runs
- Each run takes 2 hours, plus 0.5 hours to load
  - 18 runs x 1 hours = 18 hours running time
18 hour running time x € 100 hourly rate
- Totals € 1800,- running cost for >6000 proteins

Pulled emitters

In several sizes, inquire via e-mail.

25 Euro each (ex BTW)