Proteomics

Our approaches

Cutting-Edge Approaches for Experimental Workflows

Our advanced experimental workflows and quantification strategies enable precise and reliable insights into protein interactions and molecular mechanisms. From (phospho)proteomics to affinity purification techniques, we offer tailored solutions for diverse research needs. Our quantification methods, including Label-Free, SILAC, Dimethyl, and Isobaric TMT labeling, ensure accurate and scalable data analysis for a wide range of experimental conditions. Explore how our innovative approaches can elevate your research.

Approaches

Experimental workflows

We provide the following experimental workflows:

(Phospho)proteomics (with relative or absolute quantification).
Protein affinity purification using epitope tags such as GFP, HA, FLAG or Myc tag.
Peptide or DNA affinity purification (identification of proteins interacting with a specific (modified) peptide or DNA sequence of interest).
PAQMAN for the determination of apparent Kd of transcription factors for a specific sequence.

Example of an experimental workflow.

Quantification

The following quantification strategies are available:

Label Free Quantification — Comparison of 2 or more conditions in triplicates, possibility to calculate stoichiometry for stable complexes.
Stable Isotope Labeling by Amino acids in Cell culture — SILAC, only for easy-to-culture cell lines.
Dimethyl labeling — For comparison of 2 or 3 conditions.
Isobaric TMT labeling — For comparison of >5 conditions.