Olink Targeted Proteomics

Equipment

Advanced Technology for Precision Research

Discover cutting-edge tools and solutions designed to revolutionize protein analysis and molecular research. Our technology portfolio combines high-throughput capabilities with exceptional sensitivity, enabling breakthroughs in biomarker discovery and disease research. From Olink® Target panels to Biomark™ HD systems, we offer versatile, scalable solutions to meet the demands of modern science. Explore how our innovative platforms can empower your research with unmatched precision and flexibility.

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Technology

The unique technology behind our Olink® Target 96 and Target 48 panels enables high-throughput, multiplex immunoassays of proteins using minimal volumes of serum, plasma, or almost any other type of biological sample. PEA technology enables high multiplexing levels and sample throughput without compromising on data or assay-robustness.

Technology capabilities

Using PEA, our panels can provide accurate quantification below pg/mL levels even in small samples from, for example, needle biopsies or micro dialysis. This minimal sample consumption makes our precision proteomics solution especially suitable for retroactively mining precious biobanked serum samples for new multivariate biomarker signatures. Fully validated in serum & plasma, PEA is also compatible with a wide range of different sample types.

Olink® T96 panels

Each panel is focused on a specific area of disease or key biology process, targeting 92 established and/or exploratory biomarkers. All assays are rigorously quality controlled, and our validation data is made freely available. Currently there are six disease area-focus panels available: two for cardiovascular disease (Olink Target 96 Cardiovascular II and Olink Target 96 Cardiovascular III), two for oncology (Olink Target 96 Oncology II and Olink Target 96 Oncology III), as well as Olink Target 96 Neurology, Olink Target 96 Inflammation and Olink Target 96 Immuno-oncology. These are complemented by seven panels, focused on important biological processes: Olink Target 96 Cardiometabolic, Olink Target 96 Cell Regulation, Olink Target 96 Development, Olink Target 96 Immune Response, Olink Target 96 Metabolism, Olink Target 96 Neuro Exploratory and Olink Target 96 Organ Damage. For researchers working with mouse models, Olink Target 96 Mouse Exploratory is also available.

Olink® T48 panels

Olink Target 48 Cytokine and Immune Surveillance panels as well as the Mouse Cytokine panel, is the ultimate solution for cytokine studies and inflammation-related diseases. The thorough selection of biomarkers ensures that the most important proteins covering key pathways related to cytokine signaling and inflammatory processes are well covered.

Target 48 Cytokine and Immune Surveillance panels as well as the Mouse Cytokine panel enables analysis of 45 carefully selected proteins across 40 samples simultaneously from just 1 µL sample. In comparison to other products for multiple cytokine analysis, this panels offers superior sensitivity, specificity and dynamic range. Absolute quantification in standard concentration units (pg/mL) via provision of calibrators for each assay (first time this is available in a standard Olink panel) Each analyte in the panel has been validated in terms of specificity, precision, sensitivity, dynamic range, matrix effects and interference.

Olink® Flex

Olink® Flex is a customizable made-to-order product that enables the selection and combination of up to 21 human proteins into a single protein biomarker panel. Freely mix and match from ~200 pre-validated assays covering major disease areas. Olink® Flex panels offer mid-plex proteomics with unequaled flexibility and absolute quantification to cover a wide range of needs.
Mix and match 15-21 pre-validated assays from an extensive Olink library across 40 samples simultaneously from just 1 µL sample. Each assay maintains the quality, performance, specificity, and sensitivity that our other products are known for. Our internal controls remove the need to run replicate samples, and the data is reported in absolute (pg/mL) or relative (NPX) quantification.

Biomark HD

  • Scalability. Adjust throughput from 12 to 192 samples per run without changing technologies.
  • Cost savings. Decrease the number of runs and reagent volumes by more than 95% compared to using plates.
  • Flexibility. Run multiple applications and easily change markers over time.
  • Trusted performance. Use validated assays on a system that has yielded over 1,000 publications in more than 10 years of use.

Biomark™ HD sets a new standard for highthroughput real-time PCR, endpoint PCR and digital PCR, with benefits that are impossible to reproduce using other conventional PCR systems. Integrated fluidic circuit (IFC) technology saves time and money by performing thousands of reactions in nanoliter volumes on a device the size of a conventional 96-well plate.

Biomark HD, together with IFCs and the IFC Controller for loading samples and assays, streamlines workflows for applications demanding sensitivity and dynamic range at extremely high throughput, including genotyping, gene expression, copy number variation detection and digital PCR.

High-throughput detection

The system integrates thermal cycling and detection of PCR-based assays on Fluidigm IFCs. Supporting detection of up to three fluorescent dyes per assay, the system acquires data for each reaction chamber on the IFC simultaneously and can operate in either real- time or endpoint detection mode depending on the needs of the application.

IFCs

IFCs are the backbone of the Biomark HD system, supporting different configurations of samples and assays for multiple applications. For more information see the Biomark HD Flyer.

Assays

Biomark HD supports multiple commercially available chemistries including industry- proven TaqMan®, SNP Type™ and Delta Gene™ and Olink assays for genotyping, gene-expression and targeted proteomics.

Analysis software

Biomark HD is bundled with data collection and data analysis software for genotyping, gene expression and digital PCR. These robust and highly customizable tools support multiple data analysis methods and a variety of report types including amplification curves, heat maps and numeric tables. Results may be easily managed, annotated and archived.