At the UMC Utrecht we have a track record of biomarker discovery at the Luminex MultiPlex Core Facility.
Please contact Edward Knol about our services and fees.
After setting up the 194 analytes we have put much effort in finding novel markers for disease that are now implemented in routine diagnostics, such as IL-18 for systemic Juvenile Idiopathic Arthritis, TARC/CCL17 for Hodgkin Lymphoma and ratio IL-10/IL-6 for Intraocular Lymphoma. See figure 1.
Technically, we have standardized our panel and the analysis are performed in an ISO-9001 laboratory, Also pretreatment and storage of samples is standardized and applicants are informed. Importantly, we have determined the effects of different anti-coagulants in our analysis and described that serum, EDTA and heparin anti-coagulated blood result in a significant different in the markers determined as shown in a principle component analysis of 35 healthy controls. See figure 2.
For several local, national and international research groups, clinics as well as industrial partners, we have analysed numerous research materials and patient samples. For instance. within our institute we have a long time collaboration on atopic dermatitis and have described multiple diseases endotypes, described an algorithm of three biomarkers that predicts severity (pEASI, see Figure 3) and discovered a combination of 8 biomarkers that predicts local versus systemic treatment of severe atopic dermatitis patients.
Improved multiplex immunoassay performance in human plasma and synovial fluid following removal of interfering heterophilic antibodies. de Jager W, Prakken BJ, Bijlsma JW, Kuis W, Rijkers GT. J Immunol Methods. 2005 May;300(1-2):124-35.
Differential cytokine profiles in juvenile idiopathic arthritis subtypes revealed by cluster analysis.
van den Ham HJ, de Jager W, Bijlsma JW, Prakken BJ, de Boer RJ. Rheumatology (Oxford) 2009 Aug;48(8):899-905
Altered plasma adipokine levels and in vitro adipocyte differentiation in pediatric type 1 diabetes.
Verrijn Stuart AA, Schipper HS, Tasdelen I, Egan DA, Prakken BJ, Kalkhoven E, de Jager W. J Clin Endocrinol Metab. 2012 Feb;97(2):463-72.
Plasma chemokines in early wheezers predict the development of allergic asthma.
Reubsaet LL, Meerding J, de Jager W, de Kleer IM, Hoekstra MO, Prakken BJ, van Wijk F, Arets HG.
Am J Respir Crit Care Med. 2013 Oct 15;188(8):1039-40
EASI p-EASI: Utilizing a combination of serum biomarkers offers an objective measurement tool for disease severity in atopic dermatitis patients. Thijs JL, Drylewicz J, Fiechter R, Strickland I, Sleeman MA, Herath A, May RD, Bruijnzeel-Koomen CAFM, Knol EF, Giovannone B, de Bruin-Weller MS, Nierkens S, Hijnen DJ. J Allergy Clin Immunol. 2017 Dec;140(6):1703-1705.
Effect of anticoagulants on 162 circulating immune related proteins in healthy subjects.
Scholman RC, Giovannone B, Hiddingh S, Meerding JM, Malvar Fernandez B, van Dijk MEA, Tempelman MJ, Prakken BJ, de Jager W. Cytokine. 2018 Jun;106:114-124.
Early identification of atopic dermatitis patients in need of systemic immunosuppressive treatment.
Bakker DS, Drylewicz J, Nierkens S, Knol EF, Giovannone B, Delemarre EM, van der Schaft J, Balak DMW, de Bruin-Weller MS, Thijs JL. Clin Exp Allergy. 2019 Dec;49(12):1641-1644.
Galectin-9 and CXCL10 as Biomarkers for Disease Activity in Juvenile Dermatomyositis: A Longitudinal Cohort Study and Multicohort Validation. Wienke J, Bellutti Enders F, Lim J, Mertens JS, van den Hoogen LL, Wijngaarde CA, Yeo JG, Meyer A, Otten HG, Fritsch-Stork RDE, Kamphuis SSM, Hoppenreijs EPAH, Armbrust W, van den Berg JM, Hissink Muller PCE, Tekstra J, Hoogendijk JE, Deakin CT, de Jager W, van Roon JAG, van der Pol WL, Nistala K, Pilkington C, de Visser M, Arkachaisri T, Radstake TRDJ, van der Kooi AJ, Nierkens S, Wedderburn LR, van Royen-Kerkhof A, van Wijk F. Arthritis Rheumatol. 2019 Aug;71(8):1377-1390
Confirmation of multiple endotypes in atopic dermatitis based on serum biomarkers.
Bakker DS, Nierkens S, Knol EF, Giovannone B, Delemarre EM, van der Schaft J, van Wijk F, de Bruin-Weller MS, Drylewicz J, Thijs JL. J Allergy Clin Immunol. 2021 Jan;147(1):189-198.
Biomarkers in tear fluid of dupilumab-treated moderate-to-severe atopic dermatitis patients.
Achten R, Thijs J, van Luijk C, Knol E, Delemarre E, de Graaf M, Bakker D, de Boer J, van Wijk F, de Bruin-Weller M. Clin Exp Allergy. 2023 Feb;53(2):239-243.
Inflammatory markers in cerebrospinal fluid of paediatric spinal muscular atrophy patients receiving nusinersen treatment. Scheijmans FEV, Cuppen I, Zwartkruis MM, Signoria I, van Ekris C, Asselman F, Wadman RI, Knol EF, van der Pol WL, Groen EJN. Eur J Paediatr Neurol. 2023 Jan;42:34-41.
Neuroinflammatory markers at school age in preterm born children with neurodevelopmental impairments. Van der Zwart S, Knol EF, Gressens P, Koopman C, Benders M, Roze E.
Brain Behav Immun Health. 2024 May 3;38:100791
